Anemia Profile-II Test – Details & Precautions

The Anemia Profile-II is a comprehensive test panel used to diagnose different types of anemia, iron deficiency, and related blood disorders. It includes:

1. Hemogram (Complete Blood Count – CBC)
2. Iron Studies (Serum Iron, TIBC, Transferrin Saturation, Ferritin)

---

1. Hemogram (Complete Blood Count – CBC) Test

Purpose

• Measures red blood cells (RBCs), hemoglobin (Hb), hematocrit (Hct), white blood cells (WBCs), and platelets.
• Detects anemia, infections, and blood disorders.

Key Parameters Analyzed:

• Hemoglobin (Hb): Oxygen-carrying protein in RBCs.
• Hematocrit (Hct): Percentage of RBCs in blood.
• Mean Corpuscular Volume (MCV): Average size of RBCs.
• Mean Corpuscular Hemoglobin (MCH): Amount of hemoglobin per RBC.
• Red Cell Distribution Width (RDW): Variation in RBC size.

Precautions for CBC Test

✅ Fasting is NOT required.
✅ Avoid heavy exercise before the test, as it may affect WBC count.
✅ Hydrate well before giving the sample.

---

2. Iron Studies

(A) Serum Iron Test

• Measures the amount of iron in the blood.
• Low levels indicate iron deficiency anemia.

🔹 Normal Range: 60–170 mcg/dL

(B) Total Iron Binding Capacity (TIBC) Test

• Assesses the blood’s ability to bind and transport iron.
• High TIBC suggests iron deficiency anemia, while low TIBC may indicate chronic diseases.

🔹 Normal Range: 250–450 mcg/dL

(C) Transferrin Saturation

• Percentage of iron-binding sites occupied by iron in the blood.
• Low levels indicate iron deficiency, high levels may suggest iron overload.

🔹 Normal Range: 15–50%

(D) Ferritin Test

• Measures iron storage levels in the body.
• Low ferritin = Iron deficiency anemia
• High ferritin = Iron overload (Hemochromatosis) or chronic inflammation

🔹 Normal Range:

• Men: 24–336 ng/mL
• Women: 11–307 ng/mL

Precautions for Iron Studies

✅ Fasting for 8–12 hours is recommended for accurate results.
✅ Avoid iron supplements for 24 hours before the test.
✅ Best tested in the morning when iron levels are highest.

---

Final Notes:

• If iron deficiency anemia is suspected, the doctor may recommend Vitamin B12 & Folate tests.
• If ferritin is high, further tests may be needed to check for liver disease or inflammation.